Concentration of soybean lecithin affects short-term storage success of goat semen related with seminal plasma removal

Robespierre Augusto Joaquim Araújo Silva; André Mariano Batista; Lúcia Cristina Pereira Arruda; Helder Melo de Souza; Igor Henrique de Azevedo Valença Nery; Wilton Arruda Gomes; Pierre de Castro Soares; Sildivane Valcácia Silva; Maria Madalena Pessoa Guerra

doi:10.21451/1984-3143-AR2019-0012

Original Article

Concentration of soybean lecithin affects short-term storage success of goat semen related with seminal plasma removal

Robespierre Augusto Joaquim Araújo Silva; André Mariano Batista; Lúcia Cristina Pereira Arruda; Helder Melo de Souza; Igor Henrique de Azevedo Valença Nery; Wilton Arruda Gomes; Pierre de Castro Soares; Sildivane Valcácia Silva; Maria Madalena Pessoa Guerra

http://dx.doi.org/10.21451/1984-3143-AR2019-0012 Anim Reprod, vol.16, n4, p.895-901, 2019

PDF

Downloads: 2

Abstract

Abstract: The objective of this study was to investigate the need of seminal plasma removal for short-term cooling of buck semen in soybean lecithin (SL) based extender. Each pool was divided equally, and one half was subjected to centrifugation to remove seminal plasma (SP-), while the other half remained with seminal plasma (SP+). Then, both SP+ and SP- samples were diluted in two SL extenders (extender A = 1% SL; extender B = 2% SL), cooled to 5ºC and stored for 48 hours. The sperm kinetics, evaluated by CASA, and plasma membrane integrity (PMI), acrosomal integrity (ACI) and high mitochondrial membrane potential (HMMP), evaluated by epifluorescence microscopy, were determined within five minutes after reaching 5°C (T0), as well as after 24 (T24) and 48 (T48) hours of storage. Interactions (seminal plasma vs. extender vs. time;) were observed for all variables assessed. Total and progressive motility and other variables of sperm kinetics decreased after 24 hours of cooling in the SP+ group, and after 48 hours of storage, these same variables were lower in SP+/B compared to SP-/B groups. Furthermore, SP+ reduced PMI (extender B, T48), HMMP (A and B extenders, T48) and ACI (extender A, T0) compared to SP- samples. The interactions between seminal plasma and soybean lecithin phospholipids seemed to occur in a time-dependent manner. It was concluded that the removal of seminal plasma improves the quality of goat semen that was cooled in a soybean lecithin-based extender, especially when using 2% soybean lecithin.

Keywords

buck sperm, phosphatidylcholine, liquid storage semen, phospholipase A2

References

Aboagla EM, Terada T. Effects of egg yolk during the freezing step of cryopreservation on the viability of goat spermatozoa. Theriogenology. 2004;62(6):1160-72. http://dx.doi.org/10.1016/j.theriogenology.2004.01.013. PMid:15289055.

Ari UC, Daskin A. Freezing of washed Angora goat semen with extenders added bull or ram seminal plasma. J Faculty Vet Med. University of Kafkas. 2010;46:233-7.

Bousseau S, Brillard JP, Marguant-Le Guienne B, Guérin B, Camus A, Lechat M. Comparison of bacteriological qualities of various egg yolk sources and the in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or lecithin based diluentes. Theriogenology. 1998;50(5):699-706. http://dx.doi.org/10.1016/S0093-691X(98)00175-7. PMid:10734444.

Celeghini EC, Arruda RP, Andrade AF, Nascimento J, Raphael CF, Rodrigues PH. Effects that bovine sperm cryopreservation using two different extenders has on sperm membranes and chromatin. Anim Reprod Sci. 2008;104(2-4):119-31. http://dx.doi.org/10.1016/j.anireprosci.2007.02.001. PMid:17368970.

Chelucci S, Pasciu V, Succu S, Addis D, Leoni GG, Manca ME, Naitana S, Berlinguer F. Soybean lecithin-based extender preserves spermatozoa membrane integrity and fertilizing potential during goat semen cryopreservation. Theriogenology. 2015;83(6):1064-74. http://dx.doi.org/10.1016/j.theriogenology.2014.12.012. PMid:25595356.

Coloma MA, Toledano-Díaz A, López-Sebastián A, Santiago-Moreno J. The influence of washing Spanish ibex (Capra pyrenaica) sperm on the effects of cryopreservation in dependency of the photoperiod. Theriogenology. 2010;73(7):900-8. http://dx.doi.org/10.1016/j.theriogenology.2009.11.014. PMid:20092881.

De Paz P, Esteso MC, Alvarez M, Mata M, Chamorro CA, Anel L. Development of extender based on soybean lecithin for its application in liquid ram semen. Theriogenology. 2010;74(4):663-71. http://dx.doi.org/10.1016/j.theriogenology.2010.03.022. PMid:20537695.

Del Valle I, Gómez-Durán A, Holt WV, Muiño-Blanco T, Cebrián-Pérez JA. Soy lecithin interferes with mitochondrial functions in frozen-thawed ram spermatozoa. J Androl. 2012;33(4):717-25. http://dx.doi.org/10.2164/jandrol.111.014944. PMid:22134371.

Forouzanfar M, Sharafi M, Hosseini SM, Ostadhosseini S, Hajian M, Hosseini L, Abedi P, Nili N, Rahmani HR, Nasr-Esfahani MH. In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen. Theriogenology. 2010;73(4):480-7. http://dx.doi.org/10.1016/j.theriogenology.2009.10.005. PMid:20022626.

Gil J, Lundeheim N, Söderquist L, Rodriíuez-Martínez H. Influence of extender, temperature and addition of glycerol on post-thaw sperm parameters in ram semen. Theriogenology. 2003;59(5-6):1241-55. http://dx.doi.org/10.1016/S0093-691X(02)01177-9. PMid:12527072.

Küllenberg D, Taylor LA, Schneider M, Massing U. Health effects of dietary phospholipids. Lipids Health Dis. 2012;11(1):3. http://dx.doi.org/10.1186/1476-511X-11-3. PMid:22221489.

La Falci VSN, Tortorella H, Rodrigues JL, Brandelli A. Seasonal variation of goat seminal plasma proteins. Theriogenology. 2002;57(3):1035-48. http://dx.doi.org/10.1016/S0093-691X(01)00714-2. PMid:12041898.

Leboeuf B, Restall B, Salamon S. Production and storage of goat semen for artificial insemination. Anim Reprod Sci. 2000;62(1-3):113-41. http://dx.doi.org/10.1016/S0378-4320(00)00156-1. PMid:10924822.

Liu CH, Dong HB, Ma DL, Li YW, Han D, Luo MJ, Chang ZL, Tan JH. Effects of pH during liquid storage of goat semen on sperm viability and fertilizing potential. Anim Reprod Sci. 2016;164:47-56. http://dx.doi.org/10.1016/j.anireprosci.2015.11.011. PMid:26612188.

Martin G, Cagnon N, Sabido O, Sion B, Grizard G, Durand P, Levy R. Kinetics of occurrence of some features of apoptosis during the cryopreservation process of bovine spermatozoa. Hum Reprod. 2007;22(2):380-8. http://dx.doi.org/10.1093/humrep/del399. PMid:17092986.

Mata-Campuzano M, Álvarez-Rodríguez M, Álvarez M, Tamayo-Canul J, Anel L, de Paz P, Martínez-Pastor F. Post-thawing quality and incubation resilience of cryopreserved ram spermatozoa are affected by antioxidant supplementation and choice of extender. Theriogenology. 2015;83(4):520-8. http://dx.doi.org/10.1016/j.theriogenology.2014.10.018. PMid:25499089.

Mozafari MR. Nanoliposomes: preparation and analysis. Methods Mol Biol. 2010;605:29-50. http://dx.doi.org/10.1007/978-1-60327-360-2_2. PMid:20072871.

Mutalik S, Salian SR, Avadhani K, Menon J, Joshi H, Hegde AR, Kumar P, Kalthur G, Adiga SK. Liposome encapsulated soy lecithin and cholesterol can efficiently replace chicken egg yolk in human semen cryopreservation medium. Syst Biol Reprod Med. 2014;60(3):183-8. http://dx.doi.org/10.3109/19396368.2014.902521. PMid:24654557.

Oke M, Jacob JK, Paliyath G. Effect of soybean lecithin in enhancing fruit juice/sauce quality. Food Res Int. 2010;43(1):232-40. http://dx.doi.org/10.1016/j.foodres.2009.09.021.

Paulenz H, Söderquist L, Ådnøy T, Soltun K, Sæther PA, Fjellsøy KR, Berg KA. Effect of cervical and vaginal insemination with liquid semen stored at room temperature on fertility of goats. Anim Reprod Sci. 2005;86(1-2):109-17. http://dx.doi.org/10.1016/j.anireprosci.2004.06.007. PMid:15721662.

Pellicer-Rubio MT, Magallon T, Combarnous Y. Deterioration of goat sperm viability in milk extenders is due to a bulbourethral 60-kilodalton glycoprotein with triglyceride lipase activity. Biol Reprod. 1997;57(5):1023-31. http://dx.doi.org/10.1095/biolreprod57.5.1023. PMid:9369166.

Peterson K, Kappen MAPM, Ursem PJF, Nöthling JO, Colenbrander B, Gadella BM. Microscopic and flow cytometric semen assessment of Dutch AI-bucks: effect of semen processing procedures and their correlation to fertility. Theriogenology. 2007;67(4):863-71. http://dx.doi.org/10.1016/j.theriogenology.2006.11.003. PMid:17126897.

Purdy PH. A review on goat sperm cryopreservation. Small Rumin Res. 2006;63(3):215-25. http://dx.doi.org/10.1016/j.smallrumres.2005.02.015.

Qiu JH, Li YW, Xie HL, Li Q, Dong HB, Sun MJ, Gao WQ, Tan JH. Effects of glucose metabolism pathways on sperm motility and oxidative status during long-term liquid storage of goat semen. Theriogenology. 2016;86(3):839-49. http://dx.doi.org/10.1016/j.theriogenology.2016.03.005. PMid:27061367.

Roof DJ, Bowley S, Price LL, Matsas DJ. Comparison of two commercial extenders for cryopreservation of goat semen without sperm washing. Theriogenology. 2012;77(2):412-20. http://dx.doi.org/10.1016/j.theriogenology.2011.08.015. PMid:21958627.

Salmani H, Towhidi A, Zhandi M, Bahreini M, Sharafi M. In vitro assessment of soybean lecithin and egg yolk based diluents for cryopreservation of goat semen. Cryobiology. 2014;68(2):276-80. http://dx.doi.org/10.1016/j.cryobiol.2014.02.008. PMid:24548541.

Sariözkan S, Bucak MN, Tuncer PB, Taşdemir U, Kinet H, Ulutaş PA. Effect of different extenders and centrifugation/washing on postthaw microscopic-oxidative stress parameters and fertilizing ability of Angora buck sperm. Theriogenology. 2010;73(3):316-23. http://dx.doi.org/10.1016/j.theriogenology.2009.09.015. PMid:19880169.

Soares AT, Silva SV, Batista AM, Almeida FC, Nunes JF, Peixoto CA, Guerra MM. Ultrastructure evaluation of goat spermatozoa after freezing in a skim milk-based extender with Trolox supplementation. Andrologia. 2015;47(4):470-6. http://dx.doi.org/10.1111/and.12279. PMid:24698171.

Vidal AH, Batista AM, Silva ECB, Gomes WA, Pelinca MA, Silva SV, Guerra MMP. Soybean lecithin-based extender as an alternative for goat sperm cryopreservation. Small Rumin Res. 2013;109(1):47-51. http://dx.doi.org/10.1016/j.smallrumres.2012.07.022.

Xu CL, Zhou JB, Zhao BT, Lan GC, Luo MJ, Chang ZL, Sui HS, Tan JH. Liquid storage of goat semen in chemically defined extenders. Reprod Domest Anim. 2009;44(5):771-8. http://dx.doi.org/10.1111/j.1439-0531.2008.01071.x. PMid:19019073.

Zhao BT, Han D, Xu CL, Luo MJ, Chang ZL, Tan JH. Protocol optimization for long-term liquid storage of goat semen in a chemically defined extender. Reprod Domest Anim. 2009;44(6):865-72. http://dx.doi.org/10.1111/j.1439-0531.2008.01101.x. PMid:19019070.

Concentration of soybean lecithin affects short-term storage success of goat semen related with seminal plasma removal

Robespierre Augusto Joaquim Araújo Silva; André Mariano Batista; Lúcia Cristina Pereira Arruda; Helder Melo de Souza; Igor Henrique de Azevedo Valença Nery; Wilton Arruda Gomes; Pierre de Castro Soares; Sildivane Valcácia Silva; Maria Madalena Pessoa Guerra

Abstract

Keywords

References

Links

Share

Anim Reprod