Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.21451/1984-3143-AR2019-0021
Animal Reproduction (AR)
Original Article

Chlorogenic acid improves the quality of boar semen processed in Percoll

Stenia Severo Rabelo; Carla Oliveira Resende; Thais Preisser Pontelo; Bruna Resende Chaves; Bárbara Azevedo Pereira; William Eduardo da Silva; Juliano Vogas Peixoto; Luciano José Pereira; Márcio Gilberto Zangeronimo

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Abstract

Abstract: This study aimed to evaluate if the addition of chlorogenic acid (ChA) to semen extenders improves the quality of cooled boar semen processed in Percoll. The experimental design was randomized blocks (ejaculates) in a 2×3 factorial (with or without Percoll, and three antioxidant systems: a negative control, without supplementation, a positive control – vitamin E, and ChA), totaling six treatments and 12 repetitions. ChA and vitamin E (VE) were added at 4.5 mg/ml and 400 μg/ml in extender, respectively. At 0, 48 and 72h of storage at 15ºC, 80 ml insemination doses each containing 2.0 billion sperm cells were submitted to centrifugation in Percoll. The use of Percoll impaired (P<0.01) all motility patterns but decreased (P<0.01) the number of abnormal cells at 0, 48 and 72h of storage. Both VE and ChA improved (P<0.05) the total motility after Percoll processing, but only in semen stored for 48h. The same effect was not observed (P>0.05) in semen stored for 72h. ChA improved (P<0.05) the total motility of the semen stored for 72h, but this effect was not observed (P>0.05) when the semen was processed in Percoll. The antioxidants had no effect (P>0.05) on the viability and integrity of the acrosome, but ChA reduced (P<0.05) the number of abnormal cells at 0h, while VE increased the number of abnormal cells in semen stored for 72h, independent of the use of Percoll. There was no effect (P>0.05) of antioxidants or Percoll on the concentration of malondialdehyde in seminal plasma. The use of Percoll had no effect (P>0.05) on the cholesterol efflux, but ChA increased (P<0.05) this parameter at 0h and reduced (P<0.05) in the semen stored for 72h not processed with Percoll. In conclusion, the addition of ChA to semen extenders improved the quality of boar semen processed or not in Percoll.

Keywords

antioxidants, reproduction, sperm selection

References

Ahluwalia B, Holman R. Fatty acid composition of lipids of bull, boar, rabbit and human semen. J Reprod Fertil. 1969;18(3):431-7. http://dx.doi.org/10.1530/jrf.0.0180431. PMid:5788216.

Barak Y, Menezo Y, Veiga A, Elder K. A physiological replacement for polyvinylpyrrolidone (PVP) in assisted reproductive technology. Hum Fertil (Camb). 2001;4(2):99-103. http://dx.doi.org/10.1080/1464727012000199371. PMid:11591264.

Basile A, Ferrara L, Pezzo MD, Mele G, Sorbo S, Bassi P, Montesano D. Antibacterial and antioxidant activities of ethanol extract from Paullinia cupana Mart. J Ethnopharmacol. 2005;102(1):32-6. http://dx.doi.org/10.1016/j.jep.2005.05.038. PMid:16040216.

Blom E. A one-minute live-dead sperm stain by means of eosin-nigrosin. Fertil Steril. 1950;1(2):176-7. http://dx.doi.org/10.1016/S0015-0282(16)30125-X.

Breininger E, Beorlegui NB, O’Flaherty CM, Beconi MT. Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen. Theriogenology. 2005;63(8):2126-35. http://dx.doi.org/10.1016/j.theriogenology.2004.08.016. PMid:15826678.

Breininger E, Descalzo A, Rossetti L, Abramovich D, Beconi MT. Boar sperm functionality is related to α-tocopherol content after freezing-thawing. Andrologia. 2011;43(6):409-15. http://dx.doi.org/10.1111/j.1439-0272.2010.01094.x. PMid:21486419.

Brezezińska-Slebodzińska E, Slebodziński AB, Pietras B, Wieczorek G. Antioxidant effect of vitamin E and glutathione on lipid peroxidation in boar semen plasma. Biol Trace Elem Res. 1995;47(1-3):69-74. http://dx.doi.org/10.1007/BF02790102. PMid:7779577.

Cesari A, Kaiser GG, Mucci N, Mutto A, Vincenti A, Fornes MW, Alberio RH. Integrated morphophysiological assessment of two methods for sperm selection in bovine embryo production in vitro. Theriogenology. 2006;66(5):1185-93. http://dx.doi.org/10.1016/j.theriogenology.2006.03.029. PMid:16647751.

Chio KS, Tappel AL. Inactivation of ribonuclease and other enzymes by peroxidizing lipids and by malonaldehyde. Biochemistry. 1969;8(7):2827-32. http://dx.doi.org/10.1021/bi00835a020. PMid:5808335.

Dalvit GC, Cetica P, Beconi M. Effect of α-tocopherol and ascorbic acid on bovine in vitro fertilization. Theriogenology. 1998;49(3):619-27. http://dx.doi.org/10.1016/S0093-691X(98)00012-0. PMid:10732040.

Darvesh AS, Carroll RT, Bishayee A, Geldenhuys WJ, Van der Schyf CJ. Oxidative stress and Alzheimer’s disease: dietary polyphenols as potential therapeutic agents. Expert Rev Neurother. 2010;10(5):729-45. http://dx.doi.org/10.1586/ern.10.42. PMid:20420493.

De Lamirande E, Gagnon C. Reactive oxygen species and human spermatozoa. II. Depletion of adenosine triphosphate plays an important role in the inhibition of sperm motility. J Androl. 1992;13(5):379-86. PMid:1331007.

De Vos A, Nagy ZP, Van de Velde H, Joris H, Bocken G, Van Steirteghem A. Percoll gradient centrifugation can be omitted in sperm preparation for intracytoplasmic sperm injection. Hum Reprod. 1997;12(9):1980-4. http://dx.doi.org/10.1093/humrep/12.9.1980. PMid:9363717.

Guimarães ACG, Leivas FG, Santos FW, Schwengber EB, Giotto AB, Machado CIU, Gonçalves CGM, Folchini NP, Brum DS. Reduction of centrifugation force in discontinuous percoll gradients increases in vitro fertilization rates without reducing bovine sperm recovery. Anim Reprod Sci. 2014;146(3-4):103-10. http://dx.doi.org/10.1016/j.anireprosci.2014.02.016. PMid:24646635.

Henkel RR, Schill WB. Sperm preparation for ART. Reprod Biol Endocrinol. 2003;1(1):108. http://dx.doi.org/10.1186/1477-7827-1-108. PMid:14617368.

Iwasaki A, Gagnon C. Formation of reactive oxygen species in spermatozoa of infertile patients. Fertil Steril. 1992;57(2):409-16. http://dx.doi.org/10.1016/S0015-0282(16)54855-9. PMid:1735495.

Jeong Y-J, Kim M-K, Song H-J, Kang E-J, Ock S-A, Mohana Kumar B, Balasubramanian S, Rho G-J. Effect of alpha-tocopherol supplementation during boar semen cryopreservation on sperm characteristics and expression of apoptosis related genes. Cryobiology. 2009;58(2):181-9. http://dx.doi.org/10.1016/j.cryobiol.2008.12.004. PMid:19141297.

Kato Y, Nagao Y. Effect of PVP on sperm capacitation status and embryonic development in cattle. Theriogenology. 2009;72(5):624-35. http://dx.doi.org/10.1016/j.theriogenology.2009.04.018. PMid:19604569.

Le Lannou D, Blanchard Y. Nuclear maturity and morphology of human spermatozoa selected by Percoll density gradient centrifugation or swim-up procedure. J Reprod Fertil. 1988;84(2):551-6. http://dx.doi.org/10.1530/jrf.0.0840551. PMid:3199373.

Lewis SEM, Aitken RJ. DNA damage to spermatozoa has impacts on fertilization and pregnancy. Cell Tissue Res. 2005;322(1):33-41. http://dx.doi.org/10.1007/s00441-005-1097-5. PMid:15912407.

Ma H, Liu D, Wang W, Wang L, Fu B, Li Z, He X. Effect of semen extender supplementation with trehalose, vitamin C and E on post-thaw min pig sperm qualities. Cryo Letters. 2015;36(5):308-12. PMid:26574677.

Maneesh M, Jayalekshmi H. Role of reactive oxygen species and antioxidants on pathophysiology of male reproduction. Indian J Clin Biochem. 2006;21(2):80-9. http://dx.doi.org/10.1007/BF02912918. PMid:23105620.

Martín-Hidalgo D, Barón FJ, Robina A, Bragado MJ, Llera AH, García-Marín LJ, Gil MC. Inter-and intra-breed comparative study of sperm motility and viability in Iberian and Duroc boar semen during long-term storage in MR-A and XCell extenders. Anim Reprod Sci. 2013;139(1-4):109-14. http://dx.doi.org/10.1016/j.anireprosci.2013.04.001. PMid:23660365.

Matás C, Vieira L, García-Vázquez FA, Avilés-López K, López-Úbeda R, Carvajal JA, Gadea J. Effects of centrifugation through three different discontinuous Percoll gradients on boar sperm function. Anim Reprod Sci. 2011;127(1-2):62-72. http://dx.doi.org/10.1016/j.anireprosci.2011.06.009. PMid:21784589.

Melo CL, Queiroz MG, Arruda Filho AC, Rodrigues AM, de Sousa DF, Almeida JG, Pessoa OD, Silveira ER, Menezes DB, Melo TS, Santos FA, Rao VS. Betulinic acid, a natural pentacyclic triterpenoid, prevents abdominal fat accumulation in mice fed a high-fat diet. J Agric Food Chem. 2009;57(19):8776-81. http://dx.doi.org/10.1021/jf900768w. PMid:19754196.

Mendez MF, Zangeronimo MG, Rocha LG, Faria BG, Pereira BA, Fernandes CD, Chaves BR, Murgas LD, Sousa RV. Effect of the addition of IGF-I and vitamin E to stored boar semen. Animal. 2013;7(5):793-8. http://dx.doi.org/10.1017/S1751731112002285. PMid:23211508.

O’Flaherty C, De Lamirande E, Gagnon C. Positive role of reactive oxygen species in mammalian sperm capacitation: triggering and modulation of phosphorylation events. Free Radic Biol Med. 2006;41(4):528-40. http://dx.doi.org/10.1016/j.freeradbiomed.2006.04.027. PMid:16863985.

Pereira BA, Chaves BR, Teles MC, Pontelo TP, Oliveira CR, Souza RV, Rodríguez-Gil JE, Zangeronimo MG. Chlorogenic acid improves the quality of boar semen subjected to cooled storage at 15°C. Andrologia. 2018;50(5):e12978. http://dx.doi.org/10.1111/and.12978. PMid:29508428.

Pereira BA, Zangeronimo MG, Sousa RV, Teles MC, Mendez MFB, Rocha LGP. Effet de l’acide chlorogénique sur la peroxydation lipidique et la capacité antioxydante du sperme de verrat. Journées de la Recherhe Porcine. 2014;46:293-94.

Pope CE, Zhang YZ, Dresser BL. A simple staining method for evaluating acrosomal status of cat spermatozoa. J Zoo Wildl Med. 1991;22(1):87-95.

Pursel V, Johnson L, Rampacek G. Acrosome morphology of boar spermatozoa incubated before cold shock. J Anim Sci. 1972;34(2):278-83. http://dx.doi.org/10.2527/jas1972.342278x. PMid:4551736.

Sato Y, Itagaki S, Kurokawa T, Ogura J, Kobayashi M, Hirano T, Sugawara M, Iseki K. In vitro and in vivo antioxidant properties of chlorogenic acid and caffeic acid. Int J Pharm. 2011;403(1-2):136-8. http://dx.doi.org/10.1016/j.ijpharm.2010.09.035. PMid:20933071.

Satorre MM, Breininger E, Beconi MT. Cryopreservation with alpha-tocopherol and Sephadex filtration improved the quality of boar sperm. Theriogenology. 2012;78(7):1548-56. http://dx.doi.org/10.1016/j.theriogenology.2012.06.023. PMid:22925635.

Shen T, Jiang ZL, Li CJ, Hu XC, Li QW. Effect of alpha-lipoic acid on boar spermatozoa quality during freezing-thawing. Zygote. 2016;24(2):259-65. http://dx.doi.org/10.1017/S0967199415000155. PMid:26099848.

Shin BC, Huggins JW, Carraway KL. Effects of pH, concentration and aging on the malonaldehyde reaction with proteins. Lipids. 1972;7(4):229-33. http://dx.doi.org/10.1007/BF02533218. PMid:5040879.

Signorelli J, Diaz ES, Morales P. Kinases, phosphatases and proteases during sperm capacitation. Cell Tissue Res. 2012;349(3):765-82. http://dx.doi.org/10.1007/s00441-012-1370-3. PMid:22427115.

Zhou QZ, Feng CQ, Zou YG, Shu W, Li TQ, Li F, Liu CD, Mao XM. Single- and two-layer gradient centrifugation in sperm separation: comparison and appraisal. Zhonghua Nan Ke Xue. 2010;16(3):217-9. PMid:20369547.
 

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