Echotextural characteristics of the mammary gland during early lactation in two breeds of sheep varying in milk yields

Maciej Murawski; Tomasz Schwarz; Mark Jamieson; Bahareh Ahmad; Pawel Mieczyslaw Bartlewski

doi:10.21451/1984-3143-AR2019-0025

Original Article

Echotextural characteristics of the mammary gland during early lactation in two breeds of sheep varying in milk yields

Maciej Murawski; Tomasz Schwarz; Mark Jamieson; Bahareh Ahmad; Pawel Mieczyslaw Bartlewski

http://dx.doi.org/10.21451/1984-3143-AR2019-0025 Anim Reprod, vol.16, n4, p.853-858, 2019

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Abstract

Abstract: The main goal of this preliminary study was to determine and compare ultrasonographic characteristics of the mammary gland in two genotypes of ewes varying in milk productivity at 2, 3 and 4 weeks after lambing. Ultrasonographic images of the udder were obtained using the 5.0- and 7.5-MHz transducers, in axial and coronal planes, in four low milk-yielding Polish Mountain sheep and six high milk-yielding Olkuska ewes. All ultrasonograms were subjected to computerized image analyses using commercially available image analytical software (Image ProPlus^®; Media Cybernetics Inc., San Diego, CA, USA) to determine numerical pixel values (NPVs) and heterogeneity (pixel standard deviation-PSD) of the mammary gland parenchyma. During the 28-day period post-partum, the Olkuska sheep exceeded (P < 0.05) Polish Mountain ewes in milk productivity (31.6 ± 2.7 l and 25.0 ± 4.2 l, respectively; means ± SEMs) as estimated by the mean weight gains of suckling lambs. In animals examined with the 5.0-MHz transducer, mean NPVs of the mammary gland parenchyma in Olkuska ewes and mean PSD in both genotypes of ewes were lower (P < 0.05) before than after milking. In addition, PSD recorded both before and after milking were lower (P < 0.05) in the Polish Mountain compared with Olkuska breed. Mean PSD values for the mammary gland were less (P < 0.05) before than after milking in Polish Mountain ewes and they were greater (P < 0.05) in Olkuska compared with Polish Mountain ewes examined with the 7.5-MHz probe after milking. It can be concluded that milk quantity, histomorphology of the udder and ultrasound transducer frequency may all impinge on the echotextural characteristics of the mammary parenchyma in different breeds of sheep. Our observations warrant future studies of correlations between milk composition, mammary gland histophysiology and ultrasonographic image attributes of the mammary gland in ruminants.

Keywords

sheep, milk, lactation, mammary gland, ultrasonography

References

Ahmadi B, Mirshahi A, Giffin JL, Oliveira MEF, Gao L, Hahnel A, Bartlewski PM. Preliminary assessment of the quantitative relationships between testicular tissue composition and ultrasonographic image attributes in the ram. Vet J. 2013;198(1):282-5. http://dx.doi.org/10.1016/j.tvjl.2013.06.001. PMid:23850020.

Akers RM, Capuco AV, Keys JE. Mammary histology and alveolar cell differentiation during late gestation and early lactation in mammary tissue of beef and dairy heifers. Livest Sci. 2006;105(1-3):44-9. http://dx.doi.org/10.1016/j.livsci.2006.04.026.

Barbagianni MS, Gouletsou PG, Valasi I, Petridis IG, Giannenas I, Fthenakis GC. Ultrasonographic findings in the ovine udder during lactogenesis in healthy ewes or ewes with pregnancy toxaemia. J Dairy Res. 2015;1(3):1-11. http://dx.doi.org/10.1017/S0022029915000382. PMid:26130215.

Barbagianni MS, Mavrogianni VS, Vasileiou NGC, Fthenakis GC, Petridis IG. Ultrasonographic examination of the udder in sheep. Small Rumin Res. 2017;152:86-99. http://dx.doi.org/10.1016/j.smallrumres.2016.12.009.

Bartlewski PM, Sohal J, Paravinja V, Baby T, Oliveira MEF, Murawski M, Schwarz T, Zieba DA, Keisler DH. Is progesterone the key regulatory factor behind ovulation rate in sheep? Domest Anim Endocrinol. 2017;58:30-8. http://dx.doi.org/10.1016/j.domaniend.2016.06.006. PMid:27639459.

Clague JE, Roberts N, Gibson H, Edwards RH. Muscle imaging in health and disease. Neuromuscul Disord. 1995;5(3):171-8. http://dx.doi.org/10.1016/0960-8966(94)00052-B. PMid:7633181.

Fasulkov I. Ultrasonography of the mammary gland in ruminants: a review. BJVM. 2012;15:1-12.

Gebarowska D, Wierzchoś E, Murawski M, Gregoraszczuk E. Comparison of ovarian follicles (stage, number and estradiol concentration) in high fecundity Olkuska sheep, low fecundity Polish mountain sheep and their crossbreeds. Endocr Regul. 1996;30(4):195-200. PMid:10979050.

Giffin JL, Bartlewski PM, Hahnel A. Correlations among ultrasonographic and microscopic characteristics of prepubescent ram lamb testes. Exp Biol Med (Maywood). 2014;239(12):1606-18. http://dx.doi.org/10.1177/1535370214543063. PMid:25030481.

Giffin JL, Franks SE, Rodriguez-Sosa JR, Hahnel A, Bartlewski PM. A study of morphological and haemodynamic determinants of testicular echotexture characteristics in the ram. Exp Biol Med (Maywood). 2009;234(7):794-801. http://dx.doi.org/10.3181/0812-RM-364. PMid:19429851.

Lérias JR, Hernández-Castellano LE, Suárez-Trujillo A, Castro N, Pourlis A, Almeida AM. The mammary gland in small ruminants: major morphological and functional events underlying milk production-a review. J Dairy Res. 2014;81(3):304-18. http://dx.doi.org/10.1017/S0022029914000235. PMid:24901899.

Makovický P, Nagy M, Makovický P. Comparison of external udder measurements of the sheep breeds: Improved Valachian, Tsigai, Lacaune and their crosses. Chil J Agric Res. 2013;4(4):366-71. http://dx.doi.org/10.4067/S0718-58392013000400006.

Molik E, Murawski M, Bonczar G, Wierzchoś E. Effect of genotype on yield and chemical composition of sheep milk. Anim Sci Pap Rep. 2008;26:211-8.

Norms RRR. Nutrient requirements for cattle and sheep in the traditional system. 6th ed. Poland: IZ Kraków; 1993.

Nudda A, Pulina AG, Vallebella R, Bencini R, Enne G. Ultrasound technique for measuring mammary cistern size of dairy ewes. J Dairy Sci. 2000;67(1):101-6. PMid:10717848.

Olechnowicz M, Jaśkowski JM. Ultrasound examination of mammary glands in ruminants. Med Weter. 2009;65:147-50.

Paten AM. Maternal nutritional programming in the sheep: effects on postnatal growth, mammogenesis and lactation in adult ewe offspring [thesis]. Aotearoa, New Zealand: Massey University; 2014.

Ruberte J, Carretero JA, Fernández M, Navarro M, Caja G, Kirchner F, Such X. Ultrasound mammography in the lactating ewe and its correspondence to anatomical section. Small Rumin Res. 1994;13(2):199-204. http://dx.doi.org/10.1016/0921-4488(94)90097-3.

Santos V, Simplício K, Sanchez D, Coutinho L, Teixeira P, Barros F, Almeida V, Rodrigues L, Bartlewski P, Oliveira M, Feliciano M, Vicente W. B-mode and Doppler sonography of the mammary glands in dairy goats for mastitis diagnosis. Reprod Domest Anim. 2015;50(2):251-5. http://dx.doi.org/10.1111/rda.12479. PMid:25601226.

Schwarz T, Połtowicz K, Nowak J, Murawski M, Małopolska M, Andres K, Wojtysiak D, Jamieson M, Bartlewski PM. Quantitative echotextural attributes of pectoralis major muscles in broiler chickens: physicochemical correlates and effects of dietary fat source. Animals. 2019;9(6):306. http://dx.doi.org/10.3390/ani9060306.

Suárez-Trujillo A, Capote J, Argüello A, Castro N, Morales-DelaNuez A, Torres A, Morales J, Rivero MA. Effects of breed and milking frequency on udder histological structures in dairy goats. J Appl Anim Res. 2013;41(2):166-72. http://dx.doi.org/10.1080/09712119.2012.739096.

Szencziová I, Strapák P. Ultrasonography of the udder and teat in cattle: perspective measuring technique. Slovak J Anim Sci. 2012;45:96-104.

Wang YM, Fan W, Zhang K, Zhang L, Tan Z, Ma R. Comparison of transducers with different frequencies in breast contrast-enhanced ultrasound (CEUS) using SonoVue as contrast agent. Br J Radiol. 2016;89(1063):20151050. http://dx.doi.org/10.1259/bjr.20151050. PMid:27143439.

Wu D, Barrett DMW, Rawlings NC, Giffin JL, Bartlewski PM. Relationships of changes in ultrasonographic image attributes to ovulatory and steroidogenic capacity of large antral follicles in sheep. Anim Reprod Sci. 2009;116(1-2):73-84. http://dx.doi.org/10.1016/j.anireprosci.2009.01.011. PMid:19237254.

Zięba DA, Murawski M, Schwarz T, Wierzchoś E. Pattern of follicular development in high fecundity Olkuska ewes during the estrous cycle. Reprod Biol. 2002;2(1):39-58. PMid:14666161.

Echotextural characteristics of the mammary gland during early lactation in two breeds of sheep varying in milk yields

Maciej Murawski; Tomasz Schwarz; Mark Jamieson; Bahareh Ahmad; Pawel Mieczyslaw Bartlewski

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