Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.21451/1984-3143-AR2019-0090
Animal Reproduction (AR)
Original Article

Change in plasma sirtuin 1 level by injection into uterus of resveratrol in Korean cattle

Dae Hyun Kim; Jun Koo Yi; Jae Jung Ha; Dong Yep Oh; Do Yeoung Kim; Yoon seok Lee

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Abstract

Abstract: Bovine embryonic development is closely associated with mitochondrial biogenesis, which is regulated by the sirtuin 1 (SIRT1). Resveratrol, which is a type of natural phenol produced by several plants and used as a dietary supplement, is the activator of SIRT1. Although it has been reported that resveratrol increased SIRT1 level in in vitro bovine blastocysts, there are no in vivo reports on the change in the plasma SIRT1 level in cows. Therefore, we investigated the change in the level of plasma SIRT1 by injecting different concentrations of resveratrol into the uterus of Korean cattle heifer. The level of plasma SIRT1 in the 1.0 μM resveratrol-injected group was the highest among all groups (P < 0.05). Although the level of plasma SIRT1 increased on days 7, 9, and 14 in the resveratrol-injected group, the level of plasma SIRT1 in the control group decreased. When 1.0 µM resveratrol was directly injected into the uterus of cows during artificial insemination, a pregnancy rate was 21.0% higher than that in the control group. In conclusion, our results identified that the level of plasma SIRT1 was increased by direct injection of resveratrol and improved conception rate by injection into uterus of cow during artificial insemination.

Keywords

mitochondrial biogenesis, embryonic development, resveratrol, plasma SIRT1, Korean cattle

References

Baur JA, Sinclair DA. Therapeutic potential of resveratrol: the in vivo evidence. Nat Rev Drug Discov. 2006;5(6):493-506. http://dx.doi.org/10.1038/nrd2060. PMid:16732220.

Fullerton MD, Steinberg GR. SIRT1 takes a backseat to AMPK in the regulation of insulin sensitivity by resveratrol. Diabetes. 2010;59(3):551-3. http://dx.doi.org/10.2337/db09-1732. PMid:20190133.

Galeati GSM. Resveratrol from Red Grapes: an useful agent for oocyte maturation and subsequent embryonic development. Austin J In Vitro Fertili. 2015;2:1-3.

Gut P, Verdin E. Rejuvenating SIRT1 activators. Cell Metab. 2013;17(5):635-7. http://dx.doi.org/10.1016/j.cmet.2013.04.016. PMid:23663735.

Hayashi T, Ueda S, Mori M, Baba T, Abe T, Iwata H. Influence of resveratrol pretreatment on thawed bovine embryo quality and mitochondrial DNA copy number. Theriogenology. 2018;106:271-8. http://dx.doi.org/10.1016/j.theriogenology.2017.10.022. PMid:29096276.

Lavu S, Boss O, Elliott PJ, Lambert PD. Sirtuins-novel therapeutic targets to treat age-associated diseases. Nat Rev Drug Discov. 2008;7(10):841-53. http://dx.doi.org/10.1038/nrd2665. PMid:18827827.

Oktay YEO, Haci A. Effect of exogenous progesterone on success of ovsynch protocol in dairy cows with ovarian cyst. Acta Sci Vet. 2014;42(1189):1-9.

Price NL, Gomes AP, Ling AJ, Duarte FV, Martin-Montalvo A, North BJ, Agarwal B, Ye L, Ramadori G, Teodoro JS, Hubbard BP, Varela AT, Davis JG, Varamini B, Hafner A, Moaddel R, Rolo AP, Coppari R, Palmeira CM, de Cabo R, Baur JA, Sinclair DA. SIRT1 is required for AMPK activation and the beneficial effects of resveratrol on mitochondrial function. Cell Metab. 2012;15(5):675-90. http://dx.doi.org/10.1016/j.cmet.2012.04.003. PMid:22560220.

Salzano A, Albero G, Zullo G, Neglia G, Abdel-Wahab A, Bifulco G, Zicarelli L, Gasparrini B. Effect of resveratrol supplementation during culture on the quality and cryotolerance of bovine in vitro produced embryos. Anim Reprod Sci. 2014;151(3-4):91-6. http://dx.doi.org/10.1016/j.anireprosci.2014.09.018. PMid:25304491.

Sato D, Itami N, Tasaki H, Takeo S, Kuwayama T, Iwata H. Relationship between mitochondrial DNA copy number and SIRT1 expression in porcine oocytes. PLoS One. 2014;9(4):1-8. http://dx.doi.org/10.1371/journal.pone.0094488. PMid:24747689.

Singh UP, Singh NP, Singh B, Hofseth LJ, Price RL, Nagarkatti M, Nagarkatti PS. Resveratrol (trans-3,5,4′-trihydroxystilbene) induces silent mating type information regulation-1 and down-regulates nuclear transcription factor-kappaB activation to abrogate dextran sulfate sodium-induced colitis. J Pharmacol Exp Ther. 2010;332(3):829-39. http://dx.doi.org/10.1124/jpet.109.160838. PMid:19940103.

Takeo S, Kawahara-Miki R, Goto H, Cao F, Kimura K, Monji Y, Kuwayama T, Iwata H. Age-associated changes in gene expression and developmental competence of bovine oocytes, and a possible countermeasure against age-associated events. Mol Reprod Dev. 2013;80(7):508-21. http://dx.doi.org/10.1002/mrd.22187. PMid:23712640.

Takeo S, Sato D, Kimura K, Monji Y, Kuwayama T, Kawahara-Miki R, Iwata H. Resveratrol improves the mitochondrial function and fertilization outcome of bovine oocytes. J Reprod Dev. 2014;60(2):92-9. http://dx.doi.org/10.1262/jrd.2013-102. PMid:24390595.

Verdin E. AROuSing SIRT1: identification of a novel endogenous SIRT1 activator. Mol Cell. 2007;28(3):354-6. http://dx.doi.org/10.1016/j.molcel.2007.10.013. PMid:17996699.

Wang F, Tian X, Zhang L, He C, Ji P, Li Y, Tan D, Liu G. Beneficial effect of resveratrol on bovine oocyte maturation and subsequent embryonic development after in vitro fertilization. Fertil Steril. 2014;101(2):577-86. http://dx.doi.org/10.1016/j.fertnstert.2013.10.041. PMid:24314921.
 

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