Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.21451/1984-3143-AR2019-0103
Animal Reproduction (AR)
Original Article

Testicular development induced by GnRH-IS in budgerigar (Melopsittacus undulatus)

José Antonio Herrera-Barragán; Samantha Anahí Carcoba-Pérez; Juan José Pérez-Rivero; Alejandro Ávalos-Rodríguez; Ana Karen Vargas-Ibarra; Fernando Gual-Sill; Osvaldo López-Díaz

Downloads: 1
Views: 218

Abstract

Abstract: Nowadays, the third part of parrots in the world is endangered or vulnerable; an alternative for their preservation is assisted reproduction in captivity through hormonal manipulation. In birds, GnRH is the main hormone which controls reproductive physiology, it is known there are three types: GnRH-I, GnRH-II and GnRH-III, involved in the release or inhibition of luteinizing hormone and follicle stimulant hormone to control gonadal and gametic development. The objective of this study was, to evaluate the effect of administrating synthetic GnRH-I in the testicular development of Melopsittacus undulatus. Twenty-eight adult budgerigars were randomly divided in two groups: control (n=14) and treated (n=14) with a unique dose of synthetic GnRH-I. Testicular development was assessed through ultrasonography and density was evaluated with pixels. Germinal diameter and thickness of germinal epithelium were determined with histology; there were identified and countified different cellular strains in seminiferous tubules therefore spermatobioscopy. Results. Ecographic density was: control group: 76 ± 7 pixels, treated group 41 ± 3 pixels. Thickness of germinal epitellium, 51.5 ± 2.9µm and 73.1 ± 3.1µm, for control group and treated group respectively. Sperm concentration in the treated group was 300% superior than in control group. It is concluded that the administration of synthetic GnRH-I, is a viable alternative to be used as part of the assisted reproductive techniques to induce reproduction.

Keywords

ultrasonography, parakeet, male reproduction

References

Bentley GE, Kriegsfeld LJ, Osugi T, Ukena K, O’Brien S, Perfito N, Moore IT, Tsutsui K, Wingfield JC. Interactions of gonadotropin-releasing hormone (GnRH) and gonadotropin-inhibitory hormone (GnIH) in birds and mammals. J Exp Zool A Comp Exp Biol. 2006;305(9):807-14. http://dx.doi.org/10.1002/jez.a.306. PMid:16902963.

Bradshaw G, Engebretson M. 2013. Cría y tenencia de loros: impacto de su captura y cautiverio. Estados Unidos de América: Animals and Society Institute.

Calderón G, Vargas AK, Ávalos RA, Rosales MA, Herrera JA. Caracterización y criopreservación seminal de periquito australiano Melopsittacus undulatus. Aviornis. 2015;144:72-4.

Costantini V, Carraro C, Bucci FA, Simontacchi C, Lacalandra GM, Minoia P. Influence of a new slow-release GnRH analogue implant on reproduction in the Budgerigar (Melopsittacus undulatus, Shaw 1805). Anim Reprod Sci. 2009;111(2-4):289-301. http://dx.doi.org/10.1016/j.anireprosci.2008.03.010. PMid:18440734.

Hammer Ø, Harper D, Ryan P. PAST: Paleontological Statistics Software Package for Education and Data Analysis. Palaeontol Electronica. 2001;4:9.

Hänse M, Schmidt V, Schneider S, Della Volpe A, Krautwald-Junghanns ME. Comparative examination of testicular biopsy samples and influence on semen characteristics in budgerigars (Melopsittacus undulatus). J Avian Med Surg. 2008;22(4):300-9. http://dx.doi.org/10.1647/2007-057.1. PMid:19216257.

Herrera JA, Ávalos RA, Rodríguez I, Gonzales JA, Rosales AM. Técnicas de reproducción asistida en aves domésticas y silvestres. México: Universidad Autónoma Metropolitana; 2013.

Iseki Y, Shibutani M, Maeda K, Nagahara H, Fukuoka T, Matsutani S, Kashiwagi S, Tanaka H, Hirakawa K, Ohira M. A new method for evaluating tumor-infiltrating lymphocytes (TILs) in colorectal cancerusing hematoxylin and eosin (H-E)-stained tumor sections. PLoS One. 2018;13(4):e0192744. http://dx.doi.org/10.1371/journal.pone.0192744. PMid:29698402.

Lugo C. Consideraciones especiales para la reproducción de aves psitácidas nativas y exóticas. Memorias de la Conferencia Interna en Medicina y Aprovechamiento de Fauna Silvestre, Exótica y no Convencional. 2011;7:4-14.

Melnychuk VL, Cooper MW, Kirby JD, Rorie RW, Anthony NB. Use of ultrasonography to characterize ovarian status in chicken. Poult Sci. 2002;81(6):892-5. http://dx.doi.org/10.1093/ps/81.6.892. PMid:12079058.

Moxon R, Bright L, Pritchard B, Bowen IM, Souza MB, Silva LDM, England GCW. Digital image analysis of testicular and prostatic ultrasonographic echogencity and heterogeneity in dogs and the relation to semen quality. Anim Reprod Sci. 2015;160:112-9. http://dx.doi.org/10.1016/j.anireprosci.2015.07.012. PMid:26282522.

Nakane Y, Yoshimura T. Photoperiodic regulation of reproduction in vertebrates. Annu Rev Anim Biosci. 2019;7(1):173-94. http://dx.doi.org/10.1146/annurev-animal-020518-115216. PMid:30332291.

National Institutes of Health. ImageJ: image processing and analysis in JAVA [homepage on the Internet]. 2019 [cited 2019 Aug 10]. Available from: https://imagej.nih.gov/ij/index.html

Ottinger MA, Abdelnabi M, Quinn M, Golden N, Wu J, Thompson N. Reproductive consequences of EDCs in birds: ¿what do laboratory effects mean in field species? Neurotoxicol Teratol. 2002;24(1):17-28. http://dx.doi.org/10.1016/S0892-0362(01)00195-7. PMid:11836068.

Paredes H. Control hormonal de la fertilidad en animales silvestres. Memorias de la Conferencia Interna en Medicina y Aprovechamiento de Fauna Silvestre y Exotica y no Convencional. 2010;6:63-7.

Peralta M, Miazzo R. Reproducción aviar – Bases de la reproducción animal: cursos de introducción a la producción animal y producción animal I. Cordova: Facultad de Agronomia y Veterinaria, Universidad Nacional de Rio Cuatro; 2002. p. 1-11.

Perez-Rivero J, Perez-Martinez M, Aguilar-Setien A. Histometric análisis of vampire bat (Desmodus rotundus) testicles treated with coumestrol by oral route. J Appl Anim Res. 2014;2(2):208-12. http://dx.doi.org/10.1080/09712119.2013.827578.

Prophet E. Tissue processing: dehydration, clearing, and infiltration. In: Armed Forces Institute of Pathology. Laboratory Methods in Histotechnology. Washington: American Registry of Pathology; 1994. Chapter 5; p. 29-32.

Ramaswamy S, Walker WH, Aliberti P, Sethi R, Marshall GR, Smith A, Nourashrafeddin S, Belgorosky A, Chandran UR, Hedger MP, Plant TM. The testicular transcriptome associated with spermatogonia differentiation initiated by gonadotrophin stimulation in the juvenile rhesus monkey (Macaca mulatta). Hum Reprod. 2017;32(10):2088-100. http://dx.doi.org/10.1093/humrep/dex270. PMid:28938749.

SEMARNAT. Secretaria del Medio Ambiente y Recursos Naturales [home page on the internet]. Subsecretaria de Gestión para la protección Ambiental; Dirección General de Vida Silvestre; 2010 [cited 2019 Jul 3]. Available in: https://www.gob.mx/profepa/documentos/norma-oficial-mexicana-nom-059-semarnat-2010

Whelan J, Şekercioğlu H, Wenny G. Why birds matter: from economic ornithology to ecosystem services. J Ornithol. 2015;156(S1):227-38. http://dx.doi.org/10.1007/s10336-015-1229-y.
 

5e4bf7720e8825800bb85d0a animreprod Articles
Links & Downloads

Anim Reprod

Share this page
Page Sections