Combined treatment of eCG and a non-selective inhibitor of phosphodiesterases for enhancing reproductive performance in ewes
Irene Valasi, Ekaterini K. Theodosiadou, Serafeim Papadopoulos, Mariana S. Barbagianni, Apostolos Nanos, Stavros Spanos, Georgios C. Fthenakis, Stella Chadio
Abstract
The aim of this study was to evaluate the effect of the non-selective phosphodiesterase inhibitor 3- isobutyl-1-methyl-xanthine (IBMX) given concomitantly with eCG on reproductive performance of ewes. In trial 1, eighty ewes were allocated into 8 groups (AC, A1, A2, A3 and DC, D1, D2, D3). Oestruses were synchronized by progestagen sponges. At sponges removal all ewes of A groups received 400 IU of eCG. Concomitantly with eCG, ewes of groups A1, A2 or A3 received IMBX at the dose of 2.5, 5.0 or 25.0 mg, respectively. Ewes of D1, D2 or D3 groups received only IBMX at the same doses, respectively; in each case, ewes of AC or DC groups were controls (C). In trial 2, one hundred eighteen ewes were allocated into 4 groups (BC1, B1 and BC2, B2). Oestruses were synchronized by progestagen sponges. At sponges removal ewes of BC1 or B1 received 200 IU of eCG, while ewes of BC2 or B2 groups received 100 IU. At the same time only ewes of B1 or B2 groups received 2.5 mg IBMX. In both trials, 48 hours after hormonal treatment, ewes were mated. In trial 1, pregnancy or lambing rate did not differ among A or D groups (P > 0.05). Total lambs per ewe were significantly higher in A1 compared to AC, A2, A3 or D1 groups (P < 0.05), but did not differ among D groups (P > 0.05). In trial 2, pregnancy or lambing rate did not differ among B groups (P > 0.05). Total lambs per ewe were significantly higher (P < 0.05) in B1 and B2 compared to BC1 and BC2 groups, respectively. These results indicate that IBMX combined with eCG at the end of an oestrus synchronization treatment improves litter size probably by increasing ovulation rate in ewes. This latter effect seems to be mainly dependent on the amount of gonadotrophins used.
Keywords
References
Abecia JA, Forcada F, González-Bulnes A. 2012. Hormonal control of reproduction in small ruminants. Anim Reprod Sci, 130:173-179.
Abecia JA, Forcada F, González-Bulnes A. 2011. Pharmaceutical control of reproduction in sheep and goats. Vet Clin North Am Food Anim Pract, 27:67-79.
Balazi A, Sirotkin AV, Chrenek P. 2012. Activators of protein kinase A and oxytocin affect rabbit reproduction. Cent Eur J Biol, 7:973-979.
Bister JL, Noël B, Perrad B, Mandiki SN, Mbayahaga J, Paquay R. 1999. Control of ovarian follicles activity in the ewe. Domest Anim Endocrinol, 17:315-328.
Conti M, Beavo J. 2007. Biochemistry and physiology of cyclic nucleotide phosphodiesterases: essential components in cyclic nucleotide signalling. Annu Rev Biochem, 76:481-511.
Deligiannis C, Valasi I, Rekkas CA, Goulas P, Theodosiadou E, Lainas T, Amiridis GS. 2005. Synchronization of ovulation and fixed time intrauterine insemination in ewes. Reprod Domest Anim, 40:6-10.
Fthenakis GC. 2001. Effects of retention of fetal membranes on subsequent reproductive performance of dairy ewes. Theriogenology, 61:129-135.
Greco DG, Stabenfeldt GH. 2007. The endocrine system. In Cunningham, JG and Klein, BG (Eds.). Textbook of Veterinary Physiology. St Louis, USA: WB Saunders. pp. 410-427.
Levy I, Horvath A, Azevedo M, Bertollo de Alexandre R, Stratakis CA. 2011. Phosphodiesterase function and endocrine cells: links to human disease and roles in tumor development and treatment. Curr Opin Pharmacol, 11:689-697.
Losco PE, Poulet FM, Kaminska-McNamara GZ, Klein MF. 2010. Myocardial and reproductive system toxicity of SCH 351591, a selective phosphodiesterase4 inhibitor, in CD-1 mice. Toxicol Pathol, 38:568-582.
Maurel MC, Roy F, Hervé V, Bertin J, Vaiman D, Cribiu E, Manfredi E, Bouvier F, Lantier I, Boué P, Guillou F. 2003. Immune response to equine chorionic gonadotropin used for the induction of ovulation in goats and ewes. Gynecol Obstet Fertil, 31:766-769.
McKenna SD, Pietropaolo M, Tos EG, Clark A, Fischer D, Kagan D, Bao B, Chedrese PJ, Palmer S. 2005. Pharmacological inhibition of phosphodiesterase 4 triggers ovulation in follicle-stimulating hormoneprimed rats. Endocrinology, 146:208-214.
Ménchaca A, Rubianes E. 2004. New treatments associated with timed artificial insemination in small ruminants. Reprod Fertil Dev, 16:403-413.
Menzies PI. 1997. Reproductive health management problems. In Youngquist, RS (Ed.). Current therapy in large animal theriogenology. Philadelphia, USA: WB Saunders. pp. 643-649.
Ramakrishnappa N, Rajamahendran R, Lin YM, Leung PC. 2005. GnRH in non-hypothalamic reproductive tissues. Anim Reprod Sci, 88:95-113.
Rose RD, Gilchrist RB, Kelly JM, Thompson JG, Sutton-McDowall ML. 2013. Regulation of sheep oocyte maturation using cAMP modulators. Theriogenology, 79:142-148.
Sirotkin AV, Chadio S, Chrenek P, Xylouri E, Fotopouloy H. 2010a. Phosphodiesterase inhibitor 3- isobutyl-methyl-xanthine stimulates reproduction in rabbit females. Theriogenology, 74: 1321-1326.
Sirotkin AV, Chrenek P, Chadio S, Xylouri E, Fotopouloy H, Makarevich AV. 2010b. Phosphodiesterase inhibitor 3-isobutyl-methyl-xanthine affects rabbit ovaries and oviduct. Eur J Pharmacol, 643:145-151.
Sirotkin AV, Makarevich AV, Gennieser HG, Kotwica J, Hetenyi L. 2000. Effect of four cGMP analogues with different mechanisms of action on hormone release by porcine ovarian granulosa cells in vitro. Exp Clin Endocrinol Diabetes, 108:214-219.
Sirotkin AV, Makarevich AV, Grosmann R. 2010c. Protein kinases and ovarian functions. J Cell Physiol, 226:37-45.
Sirotkin AV, Nitray J, Kolena J, Bulla J. 1994. Cooperation between LH-RH and LH in the direct action on the ovary: LH-RH stimulation of LH/hCG receptors, basal and LH-induced cAMP and cGMP release by porcine granulosa cells in vitro. Cell Signal, 6:135-140.
Valasi Ι, Chadio S, Papadopoulos S, Theodosiadou E, Kalogiannis D, Mavrogianni VS, Spanos S, Fthenakis GC, Amiridis GS. 2012. Effect of combined treatment of GnRH and non-selective inhibitor of phosphodiesterase on subsequent fertility of ewes: preliminary results. Reprod Domest Anim, 47(s5):111.
van den Hurk R, Zhao J. 2005. Formation of mammalian oocytes and their growth, differentiation and maturation within ovarian follicles. Theriogenology, 63:1717-1751.
Webb R, Campbell BK, Garverick HA, Gong JG, Gutierrez CG, Armstrong DG. 1999. Molecular mechanisms regulating follicular recruitment and selection. J Reprod Fertil Suppl, 54:33-48
Facebook
Google+
Twitter
LinkedIn
Mendeley
StumbleUpon
CiteULike
Reddit
Email