Effect of honey supplementation on Egyptian buffalo semen
Mohamed Mahmoud Moustafa kandiel, Ahmed Reda Mohamed Elkhawagah
Abstract
This study testified the effect of honey supplementation (0.5-4.0%) in milk on the quality of chilled and frozen buffalo spermatozoa. Semen was chilled with/without honey and examined for motility, viability, plasma membranes integrity by hypo-osmotic swelling test (HOS) at 0, 1, 2 and 4 h. Frozen-thawed semen was examined for the same criteria beside the viability index and in vitro cleavage rate. The motility, livability and HOS of chilled semen upsurge with honey supplementation 1.0-2.0%. The normality of chilled spermatozoa was improved in the presence of 2.0-4.0% of honey at 4 h. Tail abnormalities decreased with milkhoney 0.5, 1.0 and 2.0% at 2, 1 and 4 h, respectively. Incorporation of honey in milk extenders at levels of 0.5-2.0% was associated with an enhanced postequilibration motility. The post-thawing motility showed a steady increase with honey levels. The viability index increased (P < 0.001) with milk-honey 2.0% (109.00 ± 9.91) and 4.0% (112.00 ± 14.41). In vitro cleavage rate was clearly (P 0.001) enhanced in the co-existence of milk-honey 2.0% compared with control (74.00 vs. 45.83). In the meantime, a reasonable high cleavage rate (67.00%) was encountered with milkhoney 0.5%. In conclusion, incorporation of honey in skim milk extenders is promising to enhance the characteristics and fertilizing potential of stored buffalo’s semen due to its nutritive and protective properties.
Keywords
References
Abdul-Ghani AS, Dabdoub N, Muhammad R, Abdul-Ghani R, Qazzaz M. 2008. Effect of Palestinian honey on spermatogenesis in rats. J Med Food, 11:799-802.
Aboagla EM, Terada T. 2003. Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing. Biol Reprod, 69:1245-1250.
Akhter S. 2006. Effect of milk based extenders containing different antibiotics on sperm motility, longevity, morphology, plasma membrane integrity, in vivo fertility and bacterial control of liquid semen from buffalo bulls. Rawalpindi, Pakistan: University of Arid Agriculture. Doctoral Thesis.
Akhter S, Ansari MS, Andrabi SM, Ullah N, Qayyum M. 2008. Effect of antibiotics in extender on bacterial and spermatozoal quality of cooled buffalo (Bubalus bubalis) bull semen. Reprod Domest Anim, 43:272-278.
Amer H, Mansour H, El-Sayed A, Swelum A. 2008. Influence of filtration or centrifugation of semen diluent on the viability of chilled buffalo-bull semen. Stočarstvo, 62:351-366.
Andrabi SM. 2009. Factors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermatozoa. Reprod Domest Anim, 44:552-569.
Aoyagi Y, Fukui Y, Iwazumi Y, Urakawa M, Ono H. 1990. Effects of culture systems on development of in vitro fertilized bovine ova into blastocysts. Theriogenology, 34:749-759.
Bamba K, Kojima Y, Iida I. 1968. Studies on deepfreezing of boar semen. V. The effect of honey-yolk diluent on the livability of frozen boar spermatozoa. Nihon Chikusan Gakkaiho, 39:415-421.
Bang H, Davidian M. 2010. Experimental statistics for biological sciences. Methods Mol Biol, 620:3-104.
Batellier F, Vidament M, Fauquant J, Duchamp G, Arnaud G, Yvon JM, Magistrini M. 2001. Advances in cooled semen technology. Anim Reprod Sci, 68:181- 190.
Beretta G, Granata P, Ferrero M, Orioli M, Maffei Facino R. 2005. Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics. Anal Chim Acta, 533:185-191.
Blesbois E. 2007. Current status in avian semen cryopreservation. World's Poult Sci J, 63:213-222.
Brackett BG, Oliphant G. 1975. Capacitation of rabbit spermatozoa in vitro. Biol Reprod, 12:260-274.
Bustamante Filho IC, Pederlozzi CD, Sgaravatti AM, Gregory RM, Dutra Filho CS, Jobim MIM, Mattos RC. 2009. Skim Milk-egg based semen extender compensates for non-enzymatic antioxidant activity loss during equine semen cryopreservation. Anim Reprod, 6:392-399.
Chenoweth PJ. 2002. Semen quality assessment. In: Proceedings, The Applied Reproductive Strategies in Beef Cattle Workshop, 2002, Manhattan, KS. Manhattan, KS: Kansas State University. pp. 247-254.
El-Nattat WS, El-Sheshtawy RI, El-Batawy KA, Shahba MI, ElSeadawy IE. 2016. Preservability of buffalo bull semen in tris-citrate extender enriched with bee’s honey. J Innov Pharm Biol Sci, 3:180-185.
El-Sheshtawy RI, El-Nattat WS, Sabra HA, Ali AH. 2014. Effect of honey solution on semen preservability of local breeds of cattle bulls. World Appl Sci J, 32:2076-2078.
Gadea J. 2003. Review: semen extenders used in the artificial insemination of swine. Span J Agric Res, 1:17- 27.
Iritani A, Niwa K. 1977. Capacitation of bull spermatozoa and fertilization in vitro of cattle follicular oocytes matured in culture. J Reprod Fertil, 50:119-121.
Iritani A, Kasai M, Niwa K, Song HB. 1984. Fertilization in vitro of cattle follicular oocytes with ejaculated spermatozoa capacitated in a chemically defined medium. J Reprod Fertil, 70:487-492.
Jerez R, González N, Olaciregui M, LuñoV, de Blas I, Gil L. 2016. Use of soy milk combined with different cryoprotectants for the ram semen cryopreservation. Small Rumin Res, 134:34-38.
Jerez-Ebensperger R, Gil L, Gonzales N, De Blas I. 2015. The combined use of honey, garlic (Allium sativum l.) and skimmed milk as an extender for chilling sheep semen. Cryo Letters, 36:243-251.
Kántor Z, Pitsi G, Thoen J. 1999. Glass transition temperature of honey as a function of water content as determined by differential scanning calorimetry. J Agric Food Chem, 47:2327-2330.
Mahmoud KGM. 2001. Cytogenetic studies on in-vitro fertilization in buffaloes. Giza: Faculty of Veterinary Medicine, Cairo University. PhD Thesis.
Moskaug JO, Carlsen H, Myhrstad MC, Blomhoff R. 2005. Polyphenols and glutathione synthesis regulation. Am J Clin Nutr, 81:277s-283s.
Niwa K, Ohgoda O. 1988. Synergistic effect of caffeine and heparin on in-vitro fertilization of cattle oocytes matured in culture. Theriogenology, 30:733-741. Olayemi FO, Adeniji DA, Oyeyemi MO. 2011. Evaluation of sperm motility and viability in honey - included egg yolk based extender. Global Vet, 7:19-21.
Salisbury GW, VanDemark NL, Lodge JR. 1978. Extenders and extension of unfrozen semen. In: Salisbury GW (Ed.). Physiology of Reproduction and Artificial Insemination of Cattle. San Francisco, CA: W.H. Freeman. pp. 442-493.
Sansone G, Nastri MJ, Fabbrocini A. 2000. Storage of buffalo (Bubalus bubalis) semen. Anim Reprod Sci, 62:55-76.
Sharma UD (Ed.). 1962. In Vitro Preservation of Sperms. New Delhi: Indian Council of Agricultural Research. JCAR Review Series No. 35. 58 pp. Avaliable on: http://krishikosh.egranth.ac.in/bitstream/1/2035693/1/IV RI%20B%20574.pdf.
Silva PM, Gauche C, Gonzaga LV, Costa ACO, Fett R. 2016. Honey: chemical composition, stability and authenticity. Food Chem, 196:309-323.
Warriach HM, Chohan KR. 2004. Thickness of cumulus cell layer is a significant factor in meiotic competence of buffalo oocytes. J Vet Sci, 5:247-251.
Watson PF. 2000. The causes of reduced fertility with cryopreserved semen. Anim Reprod Sci, 60/61:481-492.
Yimer N, Muhammad N, Sarsaifi K, Rosnina Y, Wahid H, Khumran AM, Kaka A. 2015. Effect of honey supplementation into Tris extender on cryopreservation of bull spermatozoa. Mal J AnimSci, 18:47-54.
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