Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.21451/1984-3143-AR896
Animal Reproduction (AR)
Original Article

Effects of oxygen concentration in IVM/IVF on marmoset monkey oocyte maturation and embryo development

Olena Y. Tkachenko, Stefka Delimitreva, Edris Wedi, Johanna U. Scheerer-Bernhard, Rodrigo R. Valle, Penelope L. Nayudu

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Abstract

The effect of oxygen (O2) concentration on in vitro development of the oocytes from antral follicles of naturally cycling common marmosets (Callithrix jacchus) has been investigated. Different O2 concentrations during in vitro maturation (20 or 8%) and during in vitro fertilization (20 or 5%) have been applied in three combinations: 8-5, 20-20, 20-5 (maturation and fertilization, respectively). Additionally, 8-20 conditions were tested in a pre-study, but since no fertilization occurred, this test group was excluded from further experiments. Oocyte maturation rate was significantly higher after IVM in 20% than in 8% O2 (MI: 91% in 20-20 vs. 78% in 8-5; MII: 69 vs. 45% in 20-5 and 50% in 8-5, P < 0.05 for all). The lowest rate of embryo development occurred after maturation under 8% O2 (59% in 8-5 vs. 95% in 20-20 and 83% in 20-5, P < 0.05 for all), strongly suggesting this low tension may produce hypoxic conditions during maturation, which damages the oocytes. In conclusion, 20% O2 was shown to be significantly superior to 8% O2 for marmoset oocyte maturation, and 5% O2 was to be preferred over 20% for IVF, based on a trend for better embryo morphology and rates of progression.

Keywords

embryo, gas environment, in vitro fertilization, in vitro maturation, marmoset monkey, oocyte, oxygen.

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