Animal Reproduction (AR)
https://animal-reproduction.org/journal/animreprod/article/5b5a607cf7783717068b47b8
Animal Reproduction (AR)
Original Article

Swine semen cooled at 5ºC with PIGPEL-5 extender: effects on semen quality in vitro and fertility estimators in vivo

M.N. Corrêa, T. Lucia Jr., I. Bianchi, E. Schmitt, J. Bordignon, D.C. Rech, I.A. Peruzzo, J.C. Deschamps

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Abstract

This study consisted of two experiments. In Experiment 1, in vitro semen quality was assessed by comparing swine semen conditioned with either PIGPEL-5 extender at 5ºC or BTS extender at 17°C. Sperm motility was similar (P > 0.05) at 24 and 48 h post-dilution for PIGPEL-5 (67.9% and 59.3%, respectively) and BTS (73.9% and 64.0%, respectively). The frequency of sperm vigor equal to 4 was higher for PIGPEL-5 (P < 0.05) than for BTS after 24 h (70.8% and 29.2%, respectively) and 48 h (87.9% and 12.1%, respectively). After a thermal stress test, the frequencies of sperm vigor equal to 3 and 4 were higher (P < 0.05) for PIGPEL-5 than for BTS in both periods, but motility was not different between extenders (P > 0.05) in any period. Acrosome integrity was lower (P < 0.05) for PIGPEL-5 than for BTS in both periods, but head and tail morphology did not differ (P > 0.05). In the hypoosmotic swelling test, the frequency of tail rolling or bending was higher (P < 0.05) for BTS than for PIGPEL-5 after 24 h (33.1% and 8.2%, respectively) and 48 h (24.3% and 7.2%, respectively). In Experiment 2, 60 pre-pubertal gilts were artificially inseminated following induction of ovulation and using semen conditioned with both extenders (n = 30, per extender). There was no difference (P > 0.05) in the number of recovered or fertilized oocytes or in the fertilization rate for BTS (6.6 ± 0.6, 6.0 ± 0.6, and 83.7% ± 4.4, respectively) and for PIGPEL-5 (4.6 ± 0.9, 4.3 ± 0.8, and 87.3% ± 6.3, respectively). These results indicate that the PIGPEL-5 extender can be used to condition swine semen at 5ºC because parameters of semen quality and in vivo fertility following its use were generally similar to those of a conventional extender at 17°C.

Keywords

swine semen, cooling, extender, semen quality, fertilization rate
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