Animal Reproduction (AR)
Animal Reproduction (AR)
Original Article

Effect of manganese on bovine sperm motility, viability, and lipid peroxidation in vitro

A.K. Bansal, G.S. Bilaspuri

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Manganese (Mn2+) is a chain-breaking antioxidant in biological systems. The objective of the present study was to determine the optimal dose of Mn2+ to reverse free radical-mediated oxidative damage on motility, viability, and lipid peroxidation (LPO) of sperm from five bulls (local crossbred cattle). Fresh semen was suspended in 2.9% sodium citrate, divided into equal fractions, and subjected to Mn2+ treatment (0, 60, 100, or 200 µM) in the presence or absence of an oxidative stress inducer (ferrous ascorbate, FeAA; comprised of 150 µM FeSO4 and 750 µM ascorbic acid). All sperm suspensions were incubated (37°C) for 2 h. Treatment with FeAA decreased motility and viability but increased lipid peroxidation. All doses of Mn2+ increased motility and viability but decreased LPO; however, 60 µM Mn2+ was most effective. For sperm motility, viability, and LPO level, there were significant main effects of bull, treatment, and interval, as well as their interactions. In conclusion, Mn2+ reduced the oxidative stress (LPO) caused by FeAA and improved sperm motility and viability under in vitro conditions as well as under induced oxidative stress.


bovine, drug effects, lipid peroxidation, Mn2+, sperm motility
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