Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2023-0052
Animal Reproduction (AR)
ORIGINAL ARTICLE

Gestational monitoring in buffalo females: detection of Neospora caninum by PCR

Raúl Miguel Reyes-Sandoval; Dora Romero-Salas; Jenny Jovanna Chaparro-Gutiérrez; Anabel Cruz-Romero; Karla María López-Hernández; Miguel Ángel Lammoglia-Villagómez; Milagros González-Hernández; Marco Antonio Alarcón-Zapata; Rebeca Isabel Vergara-Reyes

http://dx.doi.org/10.1590/1984-3143-AR2023-0052 Anim Reprod, vol.20, n4, e20230052, 2023
PDF
Downloads: 0
Views: 392

Abstract

This study establishes the serological frequency against Neospora caninum on day zero and the presence of N. caninum DNA surveyed throughout the gestation of Bubalus bubalis females in a bovine buffalo system in the central zone of the state of Veracruz, Mexico. Blood samples were taken from 11 females in 6 different sampling periods and analyzed for N. caninum antibodies detection on day zero. DNA detection by PCR was performed on all sampling periods. The gestation months of the females were recorded for five trimesters by ultrasonography, as well as births and pregnancy losses. Recorded seropositivity and positivity for agent DNA were 90.9% (95% CI 58.7-9.7) and 36.3% (95% CI 10.9-69.2), respectively, on day zero. N. caninum DNA was detected between 18.1% (95% CI 2.3-51.7) and 45.4% (95% CI 16.7-76.6) over the five trimesters of observation, with three births and three abortions recorded. The studied water buffalo population had a high presence of N. caninum antibodies; however, the detection of N. caninum DNA remained below 47% in the females. The association was only observed in the detection of DNA with pregnant females (P 0.007). Our results support the hypothesis of the resistance of water buffaloes to infection and the onset of clinical signs against infection by N. caninum even upon a high possibility of infection and reinfection described in this production system in Mexico.

Keywords

Bubalus bubalis, Mexico, abortion, stillborn, bovine buffalo system

References

Ali Z, Waheed A, Iqbal H, Siddiqui S, Parveen Z. Trans fat, fatty acids and characteristics of slaughtered buffalo waste fat by edible rendering. Buffalo Bull [serial on the Internet]. 2016 Jun [cited 2023 Sept 28];35(2):199-208. Available from: https://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/1291

Almería S. Neospora caninum and wildlife. Int Sch Res Notices. 2013;2013:947347. http://dx.doi.org/10.5402/2013/947347. PMid:27335866.

Al-Qassab SE, Reichel MP, Ellis JT. On the biological and genetic diversity in Neospora caninum. Diversity. 2010;2(3):411-38. http://dx.doi.org/10.3390/d2030411.

Auriemma C, Lucibelli MG, Borriello G, Carlo E, Martucciello A, Schiavo L, Gallo A, Bove F, Corrado F, Girardi S, Amoroso MG, Uberti BĎ, Galiero G. PCR detection of Neospora caninum in water buffalo foetal tissues. Acta Parasitol. 2014;59(1):1-4. http://dx.doi.org/10.2478/s11686-014-0201-y. PMid:24570043.

Ayinmode AB, Akanbi IM. First report of antibodies to Neospora caninum in Nigerian cattle. J Infect Dev Ctries. 2013;7(7):564-5. http://dx.doi.org/10.3855/jidc.2935. PMid:23857393.

Balhara AK, Gupta M, Singh S, Mohanty AK, Singh I. Early pregnancy diagnosis in bovines: current status and future directions. ScientificWorldJournal. 2013;2013:958540. http://dx.doi.org/10.1155/2013/958540. PMid:24382949.

Calarco L, Ellis J. Species diversity and genome evolution of the pathogenic protozoan parasite, Neospora caninum. Infect Genet Evol. 2020;84:104444. http://dx.doi.org/10.1016/j.meegid.2020.104444. PMid:32619639.

Campanile G, Neglia G, D’Occhio MJ. Embryonic and fetal mortality in river buffalo (Bubalus bubalis). Theriogenology. 2016;86(1):207-13. http://dx.doi.org/10.1016/j.theriogenology.2016.04.033. PMid:27142486.

Chaparro GJ, Olivera AM, Ramírez VN, Villar AD, Fernández SJ, Londoño PJ, Palacio BL. Neospora caninum serostatus in dairy cattle of the Northern plains of Antioquia, Colombia. Rev MVZ Córdoba. 2017;21(3):5577-83.

Dubey JP, Schares G, Ortega-Mora LM. Epidemiology and control of neosporosis and Neospora caninum. Clin Microbiol Rev. 2007;20(2):323-67. http://dx.doi.org/10.1128/CMR.00031-06. PMid:17428888.

Dubey JP. Review of Neospora caninum and neosporosis in animals. Korean J Parasitol. 2003;41(1):1-16. http://dx.doi.org/10.3347/kjp.2003.41.1.1. PMid:12666725.

García-Vázquez Z, Rosario-Cruz R, Mejía-Estrada F, Rodríguez-Vivaz I, Romero-Salas D, Fernández-Ruvalcaba M, Cruz-Vázquez C. Seroprevalence of Neospora caninum antibodies in beef cattle in three southern states of Mexico. Trop Anim Health Prod. 2009;41(5):749-53. http://dx.doi.org/10.1007/s11250-008-9247-x. PMid:19016341.

Guarino A, Fusco G, Savini G, Di Francesco G, Cringoli G. Neosporosis in water buffalo (Bubalus bubalis) in Southern Italy. Vet Parasitol. 2000;91(1-2):15-21. http://dx.doi.org/10.1016/S0304-4017(00)00239-9. PMid:10889356.

INEGI [homepage on the Internet]. Instituto Nacional de Estadística y Geografía (INEGI) 2010. Compendio de información geográfica municipal, Cotaxtla, Veracruz de Ignacio de la Llave; 2010 [cited 2023 Oct 25]. Available from: https://www.inegi.org.mx/contenidos/app/mexicocifras/datos_geograficos/30/30049.pdf

Japa O, Nuangmek A, Prakhammin K, Flynn RJ. Prevalence of vertically transmitted Neospora caninum amongst beef cattle in Phayao, Thailand. Parasitol Int. 2019;70:98-101. http://dx.doi.org/10.1016/j.parint.2019.02.008. PMid:30822533.

Macedo CAB, Macedo MFSB, Cardim ST, Paiva MCDC, Taroda A, Barros LD, Cunha IAL, Zulpo DL, Garcia JL. Neospora caninum: evaluation of vertical transmission in slaughtered dairy cows (Bos taurus). Rev Bras Parasitol Vet. 2013;22(1):13-7. http://dx.doi.org/10.1590/S1984-29612013000100004. PMid:24252950.

McInnes LM, Ryan UM, O’Handley R, Sager H, Forshaw D, Palmer DG. Diagnostic significance of Neospora caninum DNA detected by PCR in cattle serum. Vet Parasitol. 2006;142(3-4):207-13. http://dx.doi.org/10.1016/j.vetpar.2006.07.013. PMid:16934934.

Montiel-Peña T, Romero-Salas D, García-Vázquez Z, Medina-Esparza L, Cruz-Vázquez C. Neosporosis bovina en ranchos ganaderos de la zona norte del estado de Veracruz, Mexico. Trop Subtrop Agroecosystems [serial on the Internet]. 2011 [cited 2023 Sept 28];13:469-79. Available from: https://www.redalyc.org/pdf/939/93920942025.pdf

Moore DP, Konrad JL, San Martino S, Reichel MP, Cano DB, Mendez S, Spath EJL, Odeón AC, Crudeli G, Campero CM. Neospora caninum serostatus is affected by age and species variables in cohabiting water buffaloes and beef cattle. Vet Parasitol. 2014;203(3-4):259-63. http://dx.doi.org/10.1016/j.vetpar.2014.04.011. PMid:24792747.

Nasir A, Ashraf M, Khan MS, Yaqub T, Javeed A, Avais M, Akhtar F. Seroprevalence of Neospora caninum in dairy buffaloes in Lahore District, Pakistan. J Parasitol. 2011;97(3):541-3. http://dx.doi.org/10.1645/GE-2687.1. PMid:21506867.

Neverauskas CE, Nasir A, Reichel MP. Prevalence and distribution of Neospora caninum in water buffalo (Bubalus bubalis) and cattle in the northern territory of Australia. Parasitol Int. 2015;64(5):392-6. http://dx.doi.org/10.1016/j.parint.2015.05.009. PMid:25992666.

Okeoma CM, Williamson NB, Pomroy WE, Stowell KM, Gillespie L. The use of PCR to detect Neospora caninum DNA in the blood of naturally infected cows. Vet Parasitol. 2004;122(4):307-15. http://dx.doi.org/10.1016/j.vetpar.2004.06.001. PMid:15262009.

Reichel MP, McAllister MM, Nasir A, Moore DP. A review of Neospora caninum in water buffalo (Bubalus bubalis). Vet Parasitol. 2015;212(3-4):75-9. http://dx.doi.org/10.1016/j.vetpar.2015.08.008. PMid:26298507.

Reyes-Sandoval RM, Álvarez-Martínez JA, Rojas-Martínez C, Espinosa-Ayala E, García-Rubio VG, Ojeda-Carrasco JJ. Detección de Neospora caninum por PCR anidada en leucocitos de bovinos productores de leche. Ecosist Recur Agropecu. 2017;4(12):563-70. http://dx.doi.org/10.19136/era.a4n12.1288.

Reyes-Sandoval RM, Romero-Salas D, Cruz-Romero A, Chaparro-Gutiérrez JJ, Lammoglia-Villagómez MÁ, López-Hernández KM, Ojeda-Carrasco JJ. Frecuencia serológica de Neospora caninum en Unidades de Producción en zona central de Veracruz, Mexico. Rev MVZ Córdoba. 2021;26(3):e2135. http://dx.doi.org/10.21897/rmvz.2135.

Romero-Salas D, García-Vázquez Z, Montiel-Palacios F, Montiel-Peña T, Aguilar-Domínguez M, Medina-Esparza L, Cruz-Vázquez C. Seroprevalance of Neospora caninum antibodies in cattle in Veracruz, Mexico. J Anim Vet Adv. 2010;9(10):1445-51. http://dx.doi.org/10.3923/javaa.2010.1445.1451.

Salehi B, Amouei A, Dodangeh S, Daryani A, Sarvi S, Safari-Kharyeki MR, Salehi S, Hosseini SA, Hosseininejad Z. Molecular identification of Neospora caninum infection in aborted fetuses of sheep, cattle, and goats in Mazandaran Province, Northern Iran. Iran J Parasitol. 2021;16(3):483-9. http://dx.doi.org/10.18502/ijpa.v16i3.7102. PMid:34630594.

SAGARPA [homepage on the Internet]. Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación, Norma Oficial Mexicana NOM-062-ZOO-1999. Especificaciones técnicas para la producción, cuidado y uso de los animales de laboratorio. Diario Oficial de la Federación; Ciudad de México; August 22 2001 [cited 2022 June 9]. Available from: https://www.gob.mx/cms/uploads/attachment/file/203498/NOM-062-ZOO-1999_220801.pdf

Salguero-Romero JL, Romero-Salas D, Cruz-Romero A, Alonso-Díaz MA, Aguilar-Domínguez M, Sánchez-Montes S, Lammoglia-Villagómez MA, Chaparro-Gutiérrez JJ, Nogueira-Domingues L, Pérez de León AA, Dubey JP. Serosurveillance and risk factors for Neospora caninum infection in water buffaloes (B. bubalis) from central and southern Mexico. Vet Parasitol Reg Stud Reports. 2021;26:100631. http://dx.doi.org/10.1016/j.vprsr.2021.100631. PMid:34879942.

Santana OI, Cruz-Vázquez C, Medina-Esparza L, Ramos PM, Castellanos MC, Quezada GD. Neospora caninum: detección de ADN en sangre durante la primera gestación de vaquillas infectadas naturalmente. Vet Mex [serial on the Internet]. 2010 [cited 2023 Sept 28];41(2):131-7. Available from: https://www.scielo.org.mx/pdf/vetmex/v41n2/v41n2a6.pdf

Yao L, Yang N, Liu Q, Wang M, Zhang W, Qian WF, Hu YF, Ding J. Detection of Neospora caninum in aborted bovine fetuses and dam blood samples by nested PCR and ELISA and seroprevalence in Beijing and Tianjin, China. Parasitology. 2009;136(11):1251-6. http://dx.doi.org/10.1017/S0031182009990813. PMid:19660160.
 

Submitted date:
04/10/2023

Accepted date:
09/28/2023

65576edba953954cf923d622 animreprod Articles
Links & Downloads
1984-3143 (Electronic) 1806-9614 (Printed)
Anim Reprod ©2024 All rights reserved.

Anim Reprod

Share this page
Page Sections