Can centrifugation improve cryotolerance of bovine embryos produced in vitro?

Danieli Aparecida Bóbbo Moreski; Josmar Mazucheli; Fabio Luiz Bim Cavalieri; Anthony Cesar de Souza Castilho; Anne Kemmer Souza; Camila Bortoliero Costa; Marcelo Marcondes Seneda; Isabele Picada Emanuelli

doi:10.1590/1984-3143-AR2024-0123

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Can centrifugation improve cryotolerance of bovine embryos produced in vitro?

Danieli Aparecida Bóbbo Moreski; Josmar Mazucheli; Fabio Luiz Bim Cavalieri; Anthony Cesar de Souza Castilho; Anne Kemmer Souza; Camila Bortoliero Costa; Marcelo Marcondes Seneda; Isabele Picada Emanuelli

http://dx.doi.org/10.1590/1984-3143-AR2024-0123 Anim Reprod, vol.22, n4, e20240123, 2025

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Abstract

We tested the effects of centrifuging in vitro matured bovine oocytes for varying times on embryo development and cryotolerance. The oocytes were divided into four groups: control (GC) and centrifuged groups [5433 x g: G5, n = 463 (5 min); G10, n = 461 (10 min); and G15, n = 483 (15 min)]. After centrifugation, the oocytes underwent in vitro fertilization for embryo production. Two parameters were evaluated: i) embryonic development (n = 1,878), and ii) cryotolerance evaluation (survival and hatching rates; n = 303). The CG and G10 groups showed blastocyst rates of 42.25% and 45.77%, respectively, higher than those of the other groups (p = 0.02). The hatching rate was equal (p > 0.05) in CG (91.96%), G5: (87.74%), and G10: (95.73%) groups; however, it was lower in G15: 77.06% (p < 0.01). In the CG group, 65.88% of cryopreserved embryos survived, which was different (p < 0.05) from that in G5 (82.02%) and G10 (82.28%) (p > 0.05). Post-freeze hatching percentage was 74.0%, 87.7%, and 47.7%, in G5, G10, and G15, respectively, which was significantly greater than that in CG (p < 0.01; 26.8%). Post-freeze hatching percentage in only G10 matched that of the non-cryopreserved embryos CG (p = 0.06, 92%). We conclude that oocyte centrifugation for 10 minutes was efficient for in vitro embryonic development and cryopreservation of cattle embryos.

Keywords

blastocyst, bovine, cryopreservation, lipid, oocytes

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Submitted date:
10/22/2024

Accepted date:
09/10/2025

Can centrifugation improve cryotolerance of bovine embryos produced in vitro?

Danieli Aparecida Bóbbo Moreski; Josmar Mazucheli; Fabio Luiz Bim Cavalieri; Anthony Cesar de Souza Castilho; Anne Kemmer Souza; Camila Bortoliero Costa; Marcelo Marcondes Seneda; Isabele Picada Emanuelli

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