Animal Reproduction (AR)
Animal Reproduction (AR)
Original Article

Identification of neomales in South American catfish Rhamdia quelen on the basis of the sex ratio in the progeny

Luciano Augusto Weiss, Jurandir Joaquim Bernardes Júnior, Alex Pires de Oliveira Nuñer

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In the present study, we aimed to identify masculinized genotypic females of Rhamdia quelen on the basis of the sex ratio of the progeny. Masculinizing diets containing 3 levels of 17α-methyltestosterone (MT; 60, 90, or 120 mg kg-1 ) were administered to R. quelen fry. Thereafter, 5 phenotypic males from each MT treatment were crossed with normal females, and their progenies were sexed. At least 1 male from each treatment group generated progeny with a higher female:male ratio. These males, designated M60, M90, and M120, were crossed again with 3 other normal females to re-evaluate the sex ratio of the progeny. Moreover, a normal male was used as a control. Again, we found a higher female:male ratio in the progeny of M60, M90, and M120. The control male produced 2 sets of balanced progeny and 1 with a lower female:male ratio. Although these breeders did not generate all-female progeny (79.75 ± 7.20% of females), our findings indicate that M60, M90, and M120 can be considered masculinized genotypic females of R. quelen, suggesting the involvement of autosomal genes or polygenic interactions in sex determination.


17α-methyltestosterone, indirect sex reversal, silver catfish.


Baldisserotto B, Radünz J. 2004. Criação de Jundiá. , Santa Maria, RS: Ed. UFSM. 232 pp.

Barcellos LJG, Woehl VM, Wassermann GF, Quevedo RM, Ittzés I, Krieger MH. 2001. Plasma levels of cortisol and glucose in response to capture and tank transference in Rhamdia quelen (Quoy & Gaimard), a South American catfish. Aquacult Res, 32:121-123.

Baroiller JF, Guiguen Y, Fostier A. 1999. Endocrine and environmental aspects of sex differentiation in fish.

Cell Mol Life Sci, 55:910-931. Baroiller JF, D’Cotta H. 2001. Environment and sex determination in farmed fish. Comp Biochem Physiol C Toxicol Pharmacol, 130:399-409.

Cardoso AP, Radünz Neto J, Medeiros TS, Knöpker MA, Lazzari R. 2004. Criação de larvas de Jundiá (Rhamdia quelen) alimentadas com rações granuladas contendo fígados ou hidrolisados. Acta Sci Anim Sci, 26:457-462.

Desprez D, Géraz E, Hoareau MC, Mélard C, Bosc P, Baroiller JF. 2003. Production of a high percentage of male offspring with a natural androgen, 11βhydroxyandrostenedione (11βOHA4), in Florida Red tilapia. Aquaculture, 216:55-65.

Desprez D, Briand C, Hoareau MC, Mélard C, Bosc P, Baroiller JF. 2006. Study of sex ratio in progeny of a complex Oreochromis hybrid, the Florida Red tilapia. Aquaculture, 251:231-237.

Devlin RH, Nagahama Y. 2002. Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences. Aquaculture, 208:191-364.

Dias DC, Corrêa CF, Leonardo AF, Tachibana L, Romagosa E, Ranzani-Paiva MJT. 2011. Probiótico na larvicultura de Matrinxã, Brycon amazonicus. Acta Sci Anim Sci, 33:365-368.

Donaldson EM, Devlin RH. 1996. Uses of biotechnology to enhance production. In: Pennell W, Barton BA (Ed.). Developments in Aquaculture and Fisheries Science. Amsterdam: Elsevier Publishers. pp. 969-1020.

Dunham RA. 2004. Aquaculture and Fisheries Biotechnology: Genetic Approaches. Wallingford, UK: CABI. 504 pp.

El-Sayed AFM. 2006. Tilapia Culture. Cambridge, MA: CABI Publishing. 277 pp.

Fenocchio AS, Swarça AC, Cestari MM, Dias AL. 2003. Karyotypic characterization and NOR analysis by different banding techniques of Rhamdia quelen (Pisces, Pimelodidae) from the first plateau of the Iguaçu River (Brazil). Folia Biol, 51:3-4.

Feist G, Yeoh C-G, Fitzpatrick MS, Schreck CB. 1995. The production of functional sex-reversed male Rainbow trout with 17α-methyltestosterone and 11 βhydroxyandrostenedione. Aquaculture, 131:145-152.

Fracalossi DM, Zaniboni-Filho E, Meurer S. 2002. No rastro das espécies nativas. Panorama Aquicult, 12:43-49.

George T, Pandian TJ. 1996. Hormonal induction of sex reversal and progeny testing in the Zebra Cichlid Cichlasoma nigrofasciatum. J Exp Zool, 275:374-382.

Ghiraldelli L, Machado C, Fracalossi DM, ZaniboniFilho E. 2007. Desenvolvimento gonadal do jundiá, Rhamdia quelen (Teleostei, Siluriformes), em viveiros de terra, na região sul do Brasil. Acta Sci Biol Sci, 29:349-356.

Gomelsky B. 2003. Chromosome set manipulation and sex control in common carp: a review. Aquat Living Resour, 16:408-415.

Gomes LC, Golombieski JI, Gomes ARC, Baldisserotto B. 2000. Biologia do Jundiá Rhamdia quelen (Teleostei, Pimelodidae). Cienc Rural, 30:179-185.

Hunter GA, Donaldson EM, Stoss J, Baker I. 1983. Production of monosex female groups of Chinook salmon (Oncorhynchus tshawytscha) by the fertilization of normal ova with sperm from sex-reversed females. Aquaculture, 33:355-364.

Kamiya T, Kai W, Tasumi S, Oka A, Matsunaga T, Mizuno N, Fujita M, Suetake H, Suzuki S, Hosoya S, Tohari S, Brenner S, Miyadai T, Venkatesh B, Suzuki Y, Kikuchi K. 2012. A trans-species missense SNP in Amhr2 is associated with sex determination in the Tiger Pufferfish, Takifugu rubripes (fugu). PLoS Genet, 8:e1002798.

Kavumpurath S, Pandian TJ. 1994. Masculinization of fighting fish, Betta splendens Regan, using synthetic or natural androgens. Aquacult Res, 25:373-381.

Kirankumar S, Pandian TJ. 2002. Effect on growth and reproduction of hormone immersed and masculinized fighting fish Betta splendens. J Exp Zool, 293:606-616.

Kowalski RK, Sarosiek B, Demianowicz W, Judek J, Goryczko K, Dobosz S, Kuźmiński H, Demska-Zakęś K, Babiak I, Glogowski J. 2011. Quantitative characteristics of Rainbow trout, Oncorhynchus mykiss, neo-males (XX genotype) and super-males (YY genotype) sperm. Int J Biol Biomol Agric Food Biotechnol Eng, 5:315-322.

Longo RS, Nuñer APO. 2010. Temperatures for fertilization and hatching and their influence on determining the sex ratio of the Silver catfish, Rhamdia quelen. Acta Sci Biol Sci, 32:107-111.

Lu J, Luan P, Zhang X, Xue S, Peng L, Mahbooband S, Sun X. 2014. Gonadal transcriptomic analysis of Yellow catfish (Pelteobagrus fulvidraco): identification of sex-related genes and genetic markers. Physiol Genomics, 46:798-807.

Mair GC, Scott AG, Penman DJ, Beardmore JA, Skibinski DOF. 1991. Sex determination in the genus Oreochromis: 1. Sex reversal, gynogenesis and triploidy in O. niloticus (L.). Theor Appl Genet, 82:144-152.

Makino LC, Nakaghi LSO, Paes MCF, Malheiros EB, Dias-Koberstein TCR. 2009. Effectiveness of methods of sexual identification in Nile tilapia (Oreochromis niloticus) sexually reverted with hormone in diet with different diameters. Biosci J, 25:112-121.

Matsuda M, Nagahama Y, Shinomiya A, Sato T, Matsuda C, Kobayashi T, Morrey CE, Shibata N, Asakawa S, Shimizu N, Hori H, Hamaguchi S, Sakaizumi M. 2002. DMY is a Y-specific DM-domain gene required for male development in the Medaka fish. Nature, 417:559-563.

Müller-Belecke A, Hörstgen-Schwark G. 1995. Sex determination in tilapia (Oreochromis niloticus) sex ratios in homozygous gynogenetic progeny and their offspring. Aquaculture, 137:57-65.

Nakamura M, Kobayashi T, Chang XT, Nagahama Y. 1998. Gonadal sex differentiation in teleost fish. J Exp Zool, 281:362-372.

Okutsu T, Shikina S, Sakamoto T, Mochizuki M, Yoshizaki G. 2015. Successful production of functional Y eggs derived from spermatogonia transplanted into female recipients and subsequent production of YY supermales in Rainbow trout, Oncorhynchus mykiss. Aquaculture, 446:298-302.

Ospina-Álvarez N, Piferrer F. 2008. Temperaturedependent sex determination in fish revisited: prevalence, a single sex ratio response pattern, and possible effects of climate change. PloS One, 3:e2837. doi: 10.1371/journal.pone.0002837.

Pandian TJ, Sheela SG, Kavumpurath S. 1994. Endocrine sex reversal in fishes: masculinization evokes greater stress and mortality. Curr Sci, 66:240-243. Pandian TJ. 2013. Sex reversal. In: Pandian TJ. Endocrine Sex Differentiation in Fish. Boca Raton, FL: CRC Press. pp. 175-212.

Piferrer F, Donaldson EM. 1989. Gonadal differentiation in Coho salmon, Oncorhynchus kisutch, after a single treatment with androgen or estrogen at different stages during ontogenesis. Aquaculture, 77:251-262.

Piferrer F. 2001. Endocrine sex control strategies for the feminization of teleost fish. Aquaculture, 197:229-281.

Rougeot C, Jacobs B, Kestemont P, Melard C. 2002. Sex control and sex determinism study in Eurasian perch, Perca fluviatilis, by use of hormonally sexreversed male breeders. Aquaculture, 211:81-89.

Shelton WL, Rodrigues-Guerrero D, Lopes-Macias J. 1981. Factors affecting androgen sex reversal of Tilápia aurea. Aquaculture, 25:59-65.

Siegfried KR. 2010. In search of determinants: gene expression during gonadal sex differentiation. J Fish Biol, 76:1879-1902.

Silveira FS, Silva FM, Casaca JM. 2014. Desempenho Catarinense na Piscicultura de Água Doce. Florianópolis, SC: EPAGRI – CEDAP. 6 pp.

Sulis-Costa R, Jimenez JE, Weingartner M, Nuñer APO. 2013. Efeito da temperatura da água na fase inicial de vida e na proporção sexual do Jundiá. Bol Inst Pesca, 39:379-388.

Sun D, Zhang Y, Wang C, Hua X, Zhang XA, Yan J. 2013. Sox9-related signaling controls Zebrafish juvenile ovary-testis transformation. Cell Death Dis, 4:e930. doi: 10.1038/cddis.2013.456.

Tao W, Yuan J, Zhou L, Sun L, Sun Y, Yang S, Li M, Zeng S, Huang B, Wang D. 2013. Characterization of gonadal transcriptomes from Nile tilapia (Oreochromis niloticus) reveals differentially expressed genes. PLoS One, 8:e63604. doi: 10.1371/journal.pone.0063604.

Toledo-Filho SA, Forest F, Almeida-Toledo LF. 1996. Cadernos de Ictiogenética III: Biotecnologia Genética Aplicada à Piscicultura. São Paulo, SP: USP. 60 pp.

Woynarovich E, Horváth L. 1983. A Propagação Artificial de Peixes de Águas Tropicais. Brasília, DF: FAO/CODEVASF/CNPq. 225 pp.

Yano A, Guyomard R, Nicol B, Jouanno E, Quillet E, Klopp C, Cabau C, Bouchez O, Fostier A, Guiguen Y. 2012. An immune-related gene evolved into the master sex-determining gene in Rainbow trout, Oncorhynchus mykiss. Curr Biol, 22:1423-1428.

Zar JH. 2010. Biostatistical Analysis. 5th ed. Upper Saddle River, NJ:, Prentice Hall. 756 pp.

Zhang J, Chiodini R, Badr A, Zhang G. 2011. The impact of next-generation sequencing on genomics. J Genet Genomics, 38:95-109.

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